PspE (phage‐shock protein E) of Escherichia coli is a rhodanese

FEBS Letters

Koster, Margot¹  Adams, Hendrik¹  Tommassen, Jan¹  Teertstra, Wieke¹ 

[1] Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands

关键词: PspE;    Phage-shock response;    Rhodanese;    Escherichia coli;   

DOI  :  10.1016/S0014-5793(02)02695-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The psp (hage-hock rotein) operon of Escherichia coli is induced when the bacteria are infected by filamentous phage and under several other stress conditions. The physiological role of the individual Psp proteins is still not known. We demonstrate here that the last gene of the operon, pspE, encodes a thiosulfate:cyanide sulfurtransferase (EC 2.8.1.1; rhodanese). Kinetic analysis revealed that catalysis occurs via a double displacement mechanism as described for other rhodaneses. The K _ms for SSO₃ ²⁻ and CN⁻ were 4.6 and 27 mM, respectively.

【 授权许可】

Unknown