Antioxidants induce different phenotypes by a distinct modulation of signal transduction

FEBS Letters

Indaco, Stefania¹  Criniti, Vittoria¹  Borriello, Adriana¹  Della Ragione, Fulvio¹  Zappia, Vincenzo¹  Cucciolla, Valeria¹ 

[1] Department of Biochemistry and Biophysics, Medical School, Second University of Naples, Via Costantinopoli 16, 80138 Naples, Italy

关键词: Resveratrol;    Hydroxytyrosol;    Pyrrolidine dithiocarbamate;    Antioxidant;    Apoptosis;    Signal transduction;    DPE;    hydroxytyrosol;    PDTC;    pyrrolidine dithiocarbamate;   

DOI  :  10.1016/S0014-5793(02)03683-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Antioxidants are known to exert a preventive activity against degenerative diseases. Here, we investigated the mechanism of action of three antioxidants: resveratrol, which causes differentiation of HL-60 cells, and hydroxytyrosol and pyrrolidine dithiocarbamate which, in the same model system, activate apoptosis. The expression profile of hydroxytyrosol-treated cells showed the up-regulation of several genes, including c-jun and egr1. Pyrrolidine dithiocarbamate activates both genes, while resveratrol increases uniquely egr1. A selective modulation of signalling pathway explained this finding. All antioxidants up-regulate Erk1/2, while only hydroxytyrosol and pyrrolidine dithiocarbamate activate c-Jun N-terminal kinase (JNK). Since JNK induces apoptosis by Bcl-2 phosphorylation, we investigated this event. Bcl-2 phosphorylation was increased by hydroxytyrosol and pyrrolidine dithiocarbamate and not by resveratrol. Our results indicate that the different phenotypical effects of antioxidants correlate with modulation of selective transduction pathways.

【 授权许可】

Unknown