Transport mechanism and metabolism of olive oil hydroxytyrosol in Caco‐2 cells

FEBS Letters

Galletti, Patrizia¹  Maisto, Giovanna¹  Zappia, Vincenzo¹  Manna, Caterina¹  Cucciolla, Valeria¹  D'Angelo, Stefania¹ 

[1] Institute of Biochemistry of Macromolecules, Medical School, Second University of Naples, Via Costantinopoli 16, 80138 Naples, Italy

关键词: Antioxidant;    Hydroxytyrosol;    Olive oil;    Polyphenol;    Transport;    Caco-2 cell;    DPE;    hydroxytyrosol (3;    4-dihydroxyphenylethanol);    HMPE;    homovanillic alcohol (3-hydroxy-4-methoxy phenylethanol);    PE;    tyrosol (4-hydroxyphenylethanol);    PC;    pyrocatechol (1;    2-dihydroxybenzene);    L-DOPA;    3;    4-dihydroxy phenylalanine;    COMT;    catechol-O-methyltransferase;    P app;    apparent permeability;    ROS;    reactive oxygen species;   

DOI  :  10.1016/S0014-5793(00)01350-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

3,4-Dihydroxyphenylethanol (hydroxytyrosol; DPE) is the major phenolic antioxidant present in extra virgin olive oil, either in a free or esterified form. Despite its relevant biological effects, no data are available on its bioavailability and metabolism. The aim of the present study is to examine the molecular mechanism of DPE intestinal transport, using differentiated Caco-2 cell monolayers as the model system. The kinetic data demonstrate that [¹⁴C]DPE transport occurs via a passive diffusion mechanism and is bidirectional; the calculated apparent permeability coefficient indicates that the molecule is quantitatively absorbed at the intestinal level. The only labelled DPE metabolite detectable in the culture medium by HPLC (10% conversion) is 3-hydroxy-4-methoxyphenylethanol, the product of catechol-O-methyltransferase; when DPE is assayed in vitro with the purified enzyme a K _m value of 40 μM has been calculated.

【 授权许可】

Unknown