Maleimides stimulate oxygen reduction in illuminated thylakoids

FEBS Letters

Ivanov, Boris¹  Ignat'ev, Alexander¹  Khorobrykh, Sergey¹ 

[1] Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino 142290, Russia

关键词: Thylakoid;    Maleimide;    Oxygen uptake;    Superoxide radical;    Chl;    chlorophyll;    Cyt c;    cytochrome c;    DCMU;    3-(3;    4-dichlorophenyl)-1;    1-dimethylurea;    DNP-INT;    dinitrophenylether of 2-iodo-4-nitrothymol;    DTT;    1;    4-dithio-DL-threitol;    Gr D;    gramicidin D;    MDHA;    monodehydroascorbate radical;    MV;    methyl viologen (paraquat);    NEM;    N-ethylmaleimide;    PDM;    N;    N′-(1;    4-phenylene)dimaleimide;    PETC;    photosynthetic electron transport chain;    SOD;    superoxide dismutase;    9AA;    9-aminoacridine;   

DOI  :  10.1016/S0014-5793(02)03673-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

N-ethylmaleimide (NEM) and N,N′-(1,4-phenylene)dimaleimide (PDM) were discovered to stimulate light-induced oxygen uptake in isolated thylakoids, and PDM provided the same stimulation at one order less concentrations. Oxygen uptake rate increased promptly after NEM or PDM addition to thylakoids. The inhibitors of photosynthetic electron transport as well as catalase decreased this rate close to zero, whereas ascorbate increased it almost three-fold. Dithiothreitol suppressed oxygen uptake stimulated by NEM. NEM stimulated light-induced reduction of cytochrome c, and this stimulation was suppressed by superoxide dismutase. It was concluded that NEM and PDM being reduced can effectively reduce molecules O₂ producing superoxide radicals.

【 授权许可】

Unknown