Coffee reduced the production of neutrophil superoxide radical in vitro

2nd International Conference on Natural Resources and Life Sciences

生态环境科学

Susilawati, I.D.A.^1 ; Safaatin, A.^1 ; Burlakovs, J.^2

Department of Biomedical Science, Faculty of Dentistry, University of Jember, Kalimantan 37 Road Kampus, Tegalboto, Jember

68121, Indonesia^1

Faculty of Health and Life Sciences, Departemen of Biology and Environmental Science, Linnaeus University, 39182 Kalmar, Växjö

35195, Sweden^2

关键词: Antioxidant capacity;    Control groups;    Healthy humans;    In-vitro;    NBT (nitro blue tetrazolium);    Post test;    Reactive oxygen species;    Superoxide radical;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/293/1/012022/pdf
DOI  :  10.1088/1755-1315/293/1/012022

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Stimulated neutrophil is the main source of oxidants/free radicals, that might contribute to the state of oxidative stress. Coffee has been well known to have a high antioxidant capacity, therefore it was plausible to hypothesize that coffee might reduce oxidants/radicals produced by neutrophil. This study aimed to analyze effect of coffee on superoxide radical produced by neutrophil in vitro. This study was conducted experimentally using the post test only control group design. Neutrophil was isolated from healthy human peripheral blood. Superoxide radical production was analyzed by NBT (nitro blue tetrazolium) assay. Results showed that coffee significantly (P