Dynamic analysis of STAT6 signalling in living cells

FEBS Letters

Sullivan, Elaine²  Spiller, David G¹  Wilde, Geraint J.C¹  White, Michael R.H¹  Nelson, Glyn¹  Unitt, John F² 

[1] School of Biological Sciences, Life Sciences Building, University of Liverpool, Crown St., Liverpool L69 7ZB, UK;Lead Generation, Molecular Biology, AstraZeneca R and D Charnwood, Bakewell Road, Loughborough, Leicestershire LE11 5RH, UK

关键词: STAT6;    NF-κB;    Confocal microscopy;    Green fluorescent protein;    STAT6;    signal transducer and activator of transcription protein 6;    NF-κB;    nuclear factor κB;    JAK;    janus kinase;    IL-4;    interleukin 4;    TNFα;    tumour necrosis factor α;    EGFP;    enhanced green fluorescent protein;   

DOI  :  10.1016/S0014-5793(02)03672-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Functional activity of N- and C-terminal fluorescent fusion proteins between STAT6 and EGFP was demonstrated through IL-4-dependent transcriptional activation and nuclear translocation. The N-terminal (EGFP–STAT6) fusion protein appeared to be more active than the C-terminal fusion. In HEK-293 cells both fusion proteins formed fluorescent nuclear foci following IL-4 stimulation, but in HeLa cells nuclear accumulation was homogeneous. Stimulation of the NF-κB pathway through TNFα treatment, or expression of p65–EGFP fusion protein, repressed both basal STAT6-dependent transcriptional activity and the extent of activation in response to IL-4. This indicates a novel mechanism of inhibition of STAT6 signalling by NF-κB activation.

【 授权许可】

Unknown