Skeletal muscle mitochondrial free‐fatty‐acid content and membrane potential sensitivity in different thyroid states: involvement of uncoupling protein‐3 and adenine nucleotide translocase

FEBS Letters

Lanni, A²  Farina, P²  Goglia, F¹  Lombardi, A³  Moreno, M¹  Silvestri, E¹  De Lange, P² 

[1] Dipartimento di Scienze Biologiche ed Ambientali, Università degli Studi del Sannio, Via Port'Arsa 11, 82100 Benevento, Italy;Dipartimento di Scienze della Vita, Seconda Università degli Studi di Napoli, Via Vivaldi 43, 81100 Caserta, Italy;Dipartimento di Fisiologia Generale ed Ambientale, Università degli Studi di Napoli ‘Federico II’, Via Mezzocannone 8, 80134 Napoli, Italy

关键词: Mitochondrion;    Thyroid hormone;    Uncoupling protein;    Adenine-nucleotide translocase;    Fatty acid;   

DOI  :  10.1016/S0014-5793(02)03690-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of triiodothyronine (T3) on mitochondrial efficiency could be related to an increase in the concentrations of some proteins, such as uncoupling proteins (UCPs). Free fatty acids (FFA) seem to be a cofactor essential for the uncoupling activity of UCP3. In this paper, we report that the hypothyroidism–hyperthyroidism transition is accompanied by increases: (i) in the endogenous levels of mitochondrial FFA and (ii) in the sensitivity to FFA shown by the mitochondrial respiration rate and membrane potential, which correlated with the level of UCP3 protein. The level of the mRNA for adenine-nucleotide translocase-1 (ANT) was not affected by the thyroid state, while the ANT contribution to FFA-induced changes in mitochondrial uncoupling was low in the hypothyroid and euthyroid states but became more relevant in the hyperthyroid state at the highest concentration of FFA.

【 授权许可】

Unknown