Vitamin E sensitive genes in the developing rat fetal brain: a high‐density oligonucleotide microarray analysis

FEBS Letters

Roy, Sashwati¹  Sen, Chandan K¹  Khanna, Savita¹  Lado, Beatrice H¹ 

[1] Laboratory of Molecular Medicine, Departments of Surgery and Molecular and Cellular Biochemistry, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, 473 W. 12th Avenue, Columbus, OH 43210, USA

关键词: Fetus;    Pregnancy;    Diet;    Microarray;    Tocotrienol;    Tocopherol;    apoB;    apolipoprotein B;    DMT;    Data Mining Tool 2.0;    GAPDH;    glyceraldehyde-3-phosphate-dehydrogenase;    HO;    hemeoxygenase;    HMG2;    high-mobility group protein 2;    MAS;    Affymetrix Microarray Suite 4.0;    NOPP140;    nuclear phosphoprotein p130;    PKC;    protein kinase C;    RT-PCR;    reverse transcription-polymerase chain reaction;    TRF;    tocotrienol rich fraction;   

DOI  :  10.1016/S0014-5793(02)03309-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Vitamin E (tocopherols and tocotrienols) is essential for normal neurological function. Recently we have reported that the neuroprotective properties of tocotrienols are much more potent than that of the widely studied tocopherols (Sen, C.K., Khanna, S., Roy, S. and Parker, L. (2000) J. Biol. Chem. 275, 13049–13055). The objective of this study was to evaluate whether (i) oral supplementation of tocotrienols during pregnancy is bioavailable to fetal and mother brains; (ii) short-term change in dietary vitamin E levels of pregnant rats influences gene expression profile of developing fetal brains. We report that dietary tocotrienol is bioavailable to both mother and fetal brains. The enrichment is more in fetal brain tissue. Using a GeneChip microarray expression profiling approach we have identified a specific set of vitamin E sensitive genes in the developing rat fetal brain.

【 授权许可】

Unknown