Leptin receptor isoform expression in rat osteoblasts and their functional analysis

FEBS Letters

Kim, Hyun-Man¹  Ko, Jea Seung¹  Lee, Yun-Jung¹  Ju, Sung-Kyu²  You, Kwan-Hee²  Park, Jung-Hyun³ 

[1] Laboratory for the Study of Molecular Biointerface, Department of Oral Anatomy, College of Dentistry and Intellectual Biointerface Engineering Center (IBEC), BK21 HLS, Seoul National University, Yeonkun-Dong, Chongro-Ku, Seoul 110-749, South Korea;Department of Biology, College of Natural Sciences, Chungnam National University, Taejon 305-764, South Korea;Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA

关键词: Bone;    Flow cytometry;    Leptin;    Leptin receptor;    Osteoblasts;    STAT3;   

DOI  :  10.1016/S0014-5793(02)02889-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The genetic defect in producing the adipose hormone leptin results among others in a drastic increase of bone mass. The current understanding is that under normal circumstances, osteoblast activity is indirectly suppressed by a hypothalamic relay induced by leptin-signalling in the brain. To investigate whether leptin might also regulate osteoblast activity in a direct manner, expression of leptin receptors in rat osteoblasts was determined and their functionality was analyzed upon recombinant leptin treatment. Reverse transcription-PCR confirmed the expression of four among the six currently described receptor isoforms, which were also able to transduce cell signalling as shown by STAT3 phosphorylation after activation.

【 授权许可】

Unknown