FEBS Letters | |
Effect of human recombinant leptin on lipid handling by fully differentiated Caco‐2 cells | |
Stan, S1  Zoltowska, M1  Bendayan, M2  Asselin, C3  Delvin, E.E1  Levy, E1  Michaud, J1  Lambert, M1  | |
[1] Centre de recherche, Hôpital Ste-Justine, 3175 Côte Ste-Catherine, Montreal, QC, Canada HST 1C5;Département de Pathologie et Biologie Cellulaire, Université de Montréal, Montreal, QC, Canada;Département d'Anatomie et Biologie Cellulaire, Université de Sherbrooke, Sherbrooke, QC, Canada | |
关键词: Leptin; Leptin receptor; Lipid; Lipoprotein; Immunohistochemistry; Protein A-gold; | |
DOI : 10.1016/S0014-5793(01)03032-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
It has been established that leptin displays a number of effects on peripheral tissues. We have investigated the effect of the hormone on lipid synthesis, apolipoprotein biogenesis and lipoprotein secretion in Caco-2 cells. Immunocytochemistry revealed the presence of leptin receptors (Ob-Rb) on the basolateral membrane. Incubation of cells with 200 nM leptin resulted in a decreased export of triglycerides in the basolateral medium without affecting monoglyceride, diglyceride and cholesterol ester lipid classes. It also significantly reduced the output of de novo-synthesized apolipoprotein (Apo)B-100 and ApoB-48 as well as that of newly formed chylomicrons and of low-density lipoproteins. It also enhanced that of ApoA-I, ApoA-IV and ApoE. Our results support the hypothesis that leptin can affect energy balance at the gut level by reducing lipid release into the circulation.
【 授权许可】
Unknown
【 预 览 】
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