Effect of human recombinant leptin on lipid handling by fully differentiated Caco‐2 cells

FEBS Letters

Stan, S¹  Zoltowska, M¹  Bendayan, M²  Asselin, C³  Delvin, E.E¹  Levy, E¹  Michaud, J¹  Lambert, M¹ 

[1] Centre de recherche, Hôpital Ste-Justine, 3175 Côte Ste-Catherine, Montreal, QC, Canada HST 1C5;Département de Pathologie et Biologie Cellulaire, Université de Montréal, Montreal, QC, Canada;Département d'Anatomie et Biologie Cellulaire, Université de Sherbrooke, Sherbrooke, QC, Canada

关键词: Leptin;    Leptin receptor;    Lipid;    Lipoprotein;    Immunohistochemistry;    Protein A-gold;   

DOI  :  10.1016/S0014-5793(01)03032-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It has been established that leptin displays a number of effects on peripheral tissues. We have investigated the effect of the hormone on lipid synthesis, apolipoprotein biogenesis and lipoprotein secretion in Caco-2 cells. Immunocytochemistry revealed the presence of leptin receptors (Ob-Rb) on the basolateral membrane. Incubation of cells with 200 nM leptin resulted in a decreased export of triglycerides in the basolateral medium without affecting monoglyceride, diglyceride and cholesterol ester lipid classes. It also significantly reduced the output of de novo-synthesized apolipoprotein (Apo)B-100 and ApoB-48 as well as that of newly formed chylomicrons and of low-density lipoproteins. It also enhanced that of ApoA-I, ApoA-IV and ApoE. Our results support the hypothesis that leptin can affect energy balance at the gut level by reducing lipid release into the circulation.

【 授权许可】

Unknown