Human gastrin‐releasing peptide receptor mediates sustained CREB phosphorylation and transactivation in HuTu 80 duodenal cancer cells

FEBS Letters

Xiao, Dongmei¹  Qu, Xiangping¹  Weber, H.Christian¹ 

[1] Boston University School of Medicine, Section of Gastroenterology, 650 Albany Street, EBRC, Room 515, Boston, MA 02118, USA

关键词: Gastrin-releasing peptide receptor;    Cyclic AMP response element binding protein phosphorylation;    Bombesin;    Protein kinase C;    p38 mitogen-activated protein kinase;    Human cancer;    hGRP-R;    human gastrin-releasing peptide receptor;    Bn;    bombesin;    CREB;    cyclic AMP response element binding protein;    PKC;    protein kinase C;    PKA;    protein kinase A;    MAPK;    mitogen-activated protein kinase;    PMA;    phorbol 12-myristate 13-acetate;    FSK;    forskolin;   

DOI  :  10.1016/S0014-5793(02)03177-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The G protein-coupled human gastrin-releasing peptide receptor (hGRP-R) is frequently found aberrantly expressed in human cancers of the colon, stomach, and lung, and its ligand-specific activation has been implicated in cell proliferation and differentiation. Here, we demonstrated hGRP-R activation stimulated sustained cyclic AMP response element binding protein (CREB) phosphorylation and transactivation in duodenal cancer cells through a protein kinase C and partially p38 mitogen-activated protein kinase-dependent pathway. In contrast, intracellular calcium, ERK1/2, protein kinase A, and PI3 kinase were not involved. This novel signaling mechanism might be of importance for regulation of CREB-dependent gene expression in human cancer expressing functional hGRP-R.

【 授权许可】

Unknown