FEBS Letters | |
Human gastrin‐releasing peptide receptor mediates sustained CREB phosphorylation and transactivation in HuTu 80 duodenal cancer cells | |
Xiao, Dongmei1  Qu, Xiangping1  Weber, H.Christian1  | |
[1] Boston University School of Medicine, Section of Gastroenterology, 650 Albany Street, EBRC, Room 515, Boston, MA 02118, USA | |
关键词: Gastrin-releasing peptide receptor; Cyclic AMP response element binding protein phosphorylation; Bombesin; Protein kinase C; p38 mitogen-activated protein kinase; Human cancer; hGRP-R; human gastrin-releasing peptide receptor; Bn; bombesin; CREB; cyclic AMP response element binding protein; PKC; protein kinase C; PKA; protein kinase A; MAPK; mitogen-activated protein kinase; PMA; phorbol 12-myristate 13-acetate; FSK; forskolin; | |
DOI : 10.1016/S0014-5793(02)03177-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The G protein-coupled human gastrin-releasing peptide receptor (hGRP-R) is frequently found aberrantly expressed in human cancers of the colon, stomach, and lung, and its ligand-specific activation has been implicated in cell proliferation and differentiation. Here, we demonstrated hGRP-R activation stimulated sustained cyclic AMP response element binding protein (CREB) phosphorylation and transactivation in duodenal cancer cells through a protein kinase C and partially p38 mitogen-activated protein kinase-dependent pathway. In contrast, intracellular calcium, ERK1/2, protein kinase A, and PI3 kinase were not involved. This novel signaling mechanism might be of importance for regulation of CREB-dependent gene expression in human cancer expressing functional hGRP-R.
【 授权许可】
Unknown
【 预 览 】
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