Expression and electrophysiological identification of the receptor for bombesin and gastrin‐releasing peptide in Xenopus laevis oocytes injected with polyA+ RNA from rat brain

FEBS Letters

Morley, Steven D.¹  Meyerhof, Wolfgang¹  Richter, Dietmar¹ 

[1] Institut für Zellbiochemie und klinische Neurobiologie, Universitäts-Krankenhaus Eppendorf, Martinistr. 52, 2000 Hamburg 20, FRG

关键词: Bombesin receptor;    Gastrin-releasing peptide receptor;    Thyrotropin-releasing hormone receptor;    Serotonin receptor;    mRNA;    Microinjection;    Voltage-clamped oocyte;    (Rat brain);   

DOI  :  10.1016/0014-5793(88)80555-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The receptor for bombesin and the related peptide, gastrin-releasing peptide (GRP) has been induced in frog oocytes by injection of polyA⁺ RNA from rat brain. The primed oocytes responded to peptides of the bombesin family (GRP, neuromedin C of bombesin) by showing dose-dependent oscillations in membrane currents as recorded by the voltage-clamp method. The induced membrane changes were suppressed when oocytes were pretreated with a bombesin-receptor antagonist.

【 授权许可】

Unknown