Organization and chromosomal localization of the gene for the human bombesin receptor subtype expressed in pregnant uterus

FEBS Letters

Gorbulev, Valentin²  Akhundova, Aida²  Fahrenholz, Falk²  Grzeschik, K.-H.¹ 

[1] Medizinisches Zentrum für Humangenetik, Bahnhofstraße 7a, 35037 Marburg, Germany;Max Planck Institut für Biophysik, Kennedy Allee 70, 60596 Frankfurt, Germany

关键词: Bombesin;    Bombesin receptor;    Chromosomal localization;   

DOI  :  10.1016/0014-5793(94)80150-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The gene encoding the human homologue of the guinea pig uterine bombesin receptor [(1992) Eur. J. Biochem. 208,405] was isolated from a genomic lambda library by the PCR/homology screening approach. The gene spans more than 4 kb and consists of 3 exons and 2 introns. The deduced amino acid sequence shows about 86% identity to that of guinea pig bombesin receptor. This subtype of bombesin receptor is expressed in the pregnant uterus and in two human tumour cell lines, T47D (ductal breast carcinoma) and A431 (epidermal carcinoma). PCR analysis of genomic DNA from human-mouse cell hybrids allows the cloned gene to be localized to the region q26–q28 on chromosome X.

【 授权许可】

Unknown