Interplay between ascorbic acid and lipophilic antioxidant defences in chloroplasts of water‐stressed Arabidopsis plants

FEBS Letters

Munné-Bosch, Sergi¹  Alegre, Leonor¹ 

[1] Departament de Biologia Vegetal, Facultat de Biologia, Universitat de Barcelona, Avinguda Diagonal 645, E-08028 Barcelona, Spain

关键词: Antioxidant;    Ascorbic acid;    α-Tocopherol;    Carotenoid;    Chloroplast;    vtc-1 mutant;    AOS;    active oxygen species;    Asc;    ascorbic acid;    Asct;    total ascorbic acid (reduced plus oxidized);    α-Toc;    α-tocopherol;    β-Car;    β-carotene;    Chl;    chlorophyll a+b;    Dha;    dehydroascorbic acid;    DPS;    de-epoxidation state of the xanthophyll cycle;    Fv/Fm;    maximum efficiency of photosystem II photochemistry;    IR;    irrigated plants;    MDA;    malondialdehyde;    RWC;    relative leaf water content;    WS;    water-stressed plants;   

DOI  :  10.1016/S0014-5793(02)03041-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effects of low ascorbic acid (Asc) on lipophilic antioxidant defences and lipid peroxidation in chloroplasts were evaluated in the vtc-1 mutant of Arabidopsis thaliana, which had an Asc deficiency in chloroplasts of ca. 60%. Although low Asc did not cause oxidative stress in optimal growth conditions, it increased malondialdehyde levels in chloroplasts by ca. 60%, and reduced α-tocopherol and β-carotene by ca. 85% and 40%, respectively, in water-stressed (WS) mutants. These results are indicative of the interplay between Asc and lipophilic antioxidants in chloroplasts of WS plants in vivo, and show that Asc contributes to the protection of thylakoid membrane lipids from oxidation in stressed plants.

【 授权许可】

Unknown