Mechano‐chemical effects of Ca2+ in cross‐linked troponin‐C films

FEBS Letters

Bukatina, Anna E³  Gusev, Nikolai B¹  Morozov, Victor N²  Sieck, Gary C³ 

[1] Department of Biochemistry, School of Biology, Lomonosov Moscow State University, Moscow 119899, Russia;Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia;Departments of Anesthesiology, and Physiology and Biophysics, Mayo Foundation, 200 First Street SW, Rochester, MN 55905, USA

关键词: Troponin C;    Calcium;    Calcium-binding protein;    Conformational change;    Cross-linking;    Protein film;    TnC;    troponin C;   

DOI  :  10.1016/S0014-5793(02)03013-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Changes in troponin C (TnC) conformation upon Ca²⁺ binding forms the basis for regulatory and structural functions of TnC molecules. In the present study, Ca²⁺-induced conformational changes in TnC were observed by mechanical measurements. TnC films were prepared by drying or electrospraying TnC solutions, cross-linked with glutaraldehyde, and isometric tension and stiffness measured as a function of pCa. An increase in Ca²⁺ from a pCa of 9 to 4 induced large-scale mechanical changes in the TnC films causing several percent shrinkage of the unloaded films. This shrinkage could be partially assigned to Ca²⁺ binding to the Ca²⁺/Mg²⁺ sites of TnC.

【 授权许可】

Unknown