FEBS Letters | |
Mitochondrial ATP synthase F1‐β‐subunit is a calcium‐binding protein | |
Hubbard, Michael J.1  McHugh, Nicola J.1  | |
[1] Department of Biochemistry, University of Otago, P.O. Box 56, Dunedin, New Zealand | |
关键词: Calcium; ATP synthase; Mitochondria; Calcium-binding protein; Enamel cell; Methionine sulfoxide; F1-β-subunit; β-subunit of the F1-complex from mitochondrial ATP synthase; MALDI-TOF-MS; matrix-assisted; laser desorption ionization; time-of-flight mass spectrometry; Rt; retention time; | |
DOI : 10.1016/0014-5793(96)00767-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Mitochondrial ATP synthase is responsive to changes in cytosolic calcium concentration, but the regulatory mechanisms are unclear. Here we identified a major 52 kDa calcium-binding protein in rat enamel cells as the mitochondrial ATP synthase F1-β-subunit. The F1-β-subunit behaved as a low affinity and moderate capacity calcium-binding protein during comparative 45Ca overlay analyses. Equivalent behavior was shown by the F1-β-subunit from rat liver mitochondria, but not by the homologous F1-α-subunit, supporting the specificity of calcium binding. Evidence that the catalytic F1-β-subunit binds calcium specifically introduces new mechanistic possibilities for regulating ATP synthase, and thereby coordinating ATP production with demand for ATP-fuelled calcium pump activity.
【 授权许可】
Unknown
【 预 览 】
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RO201912020303104ZK.pdf | 756KB | download |