Mitochondrial ATP synthase F1‐β‐subunit is a calcium‐binding protein

FEBS Letters

Hubbard, Michael J.¹  McHugh, Nicola J.¹ 

[1] Department of Biochemistry, University of Otago, P.O. Box 56, Dunedin, New Zealand

关键词: Calcium;    ATP synthase;    Mitochondria;    Calcium-binding protein;    Enamel cell;    Methionine sulfoxide;    F1-β-subunit;    β-subunit of the F1-complex from mitochondrial ATP synthase;    MALDI-TOF-MS;    matrix-assisted;    laser desorption ionization;    time-of-flight mass spectrometry;    Rt;    retention time;   

DOI  :  10.1016/0014-5793(96)00767-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mitochondrial ATP synthase is responsive to changes in cytosolic calcium concentration, but the regulatory mechanisms are unclear. Here we identified a major 52 kDa calcium-binding protein in rat enamel cells as the mitochondrial ATP synthase F₁-β-subunit. The F₁-β-subunit behaved as a low affinity and moderate capacity calcium-binding protein during comparative ⁴⁵Ca overlay analyses. Equivalent behavior was shown by the F₁-β-subunit from rat liver mitochondria, but not by the homologous F₁-α-subunit, supporting the specificity of calcium binding. Evidence that the catalytic F₁-β-subunit binds calcium specifically introduces new mechanistic possibilities for regulating ATP synthase, and thereby coordinating ATP production with demand for ATP-fuelled calcium pump activity.

【 授权许可】

Unknown