A polygalacturonase of animal origin isolated from the root‐knot nematode Meloidogyne incognita 1

FEBS Letters

Jaubert, Stéphanie¹  Laffaire, Jean-Baptiste¹  Rosso, Marie-Noëlle¹  Abad, Pierre¹ 

[1] INRA, Unité Interactions Plantes-Microorganismes et Santé Végétale, 123 Boulevard Francis Meilland, P.O. Box 2078, 60606 Antibes, France

关键词: Nematode;    Pectin;    Polygalacturonase;    Stylet secretion;    J2s;    second stage juveniles;    PG;    polygalacturonase;    EST;    expressed sequence tag;    cds;    coding sequence;    PGA;    polygalacturonic acid;   

DOI  :  10.1016/S0014-5793(02)02906-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The first animal polygalacturonase (PG, EC 2.1.15) encoding cDNA, Mi-pg-1, was cloned from the plant parasitic nematode Meloidogyne incognita. The enzymatic activity of MI-PG-1 was confirmed after heterologous expression in Escherichia coli. The presence of a predicted signal peptide on the MI-PG-1 sequence together with the specific localization of the transcripts of the Mi-pg-1 gene in the oesophageal glands of infective juveniles imply that MI-PG-1 could be secreted into plant tissues. The potential role of MI-PG-1 in parasitism is discussed.

【 授权许可】

Unknown