Metabolic control analysis of anaerobic glycolysis in human hibernating myocardium replaces traditional concepts of flux control

FEBS Letters

Elsässer, Albrecht⁴  Nef, Holger⁴  Vogt, Achim M.³  Poolman, Mark¹  Fell, David A.¹  Kübler, Wolfgang³  Schaper, Jutta² 

[1] School of Molecular and Biological Sciences, Oxford Brookes University, Headington, Oxford OX3 0BP, UK;Department of Experimental Cardiology, Max-Planck-Institute for Physiological and Clinical Research (W.G. Kerckhoff-Institute), D-61231 Bad Nauheim, Germany;Department of Cardiology, University of Heidelberg, Bergheimer Straße 58, D-69115 Heidelberg, Germany;Department of Cardiology, University of Freiburg/Br., D-79106 Freiburg, Germany

关键词: Glycolysis;    Energy metabolism;    Metabolic regulation;    Ischemia;    Hibernating myocardium;    G6P;    glucose 6-phosphate;    F6P;    fructose 6-phosphate;    F1;    6P2;    fructose 1;    6-bisphosphate;    GA3P;    glyceraldehyde 3-phosphate;    DHAP;    dihydroxyacetone phosphate;    2PG;    2-phosphoglyceric acid;    PEP;    phosphoenolpyruvate;    Pyr;    pyruvate;    Lac;    lactate;    GP-iso;    glucosephosphate isomerase;    PFK-1;    phosphofructokinase 1;    GAPDH;    glyceraldehyde dehydrogenase;    PGK;    phosphoglycerate kinase;    PGM;    phosphoglycerate mutase;    Eno;    enolase;    PK;    pyruvate kinase;    LDH;    lactate dehydrogenase;   

DOI  :  10.1016/S0014-5793(02)02632-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Myocardial hibernation represents an adaptation to sustained ischemia to maintain tissue vitality during severe supply–demand imbalance which is characterized by an increased glucose uptake. To elucidate this adaptive protective mechanism, the regulation of anaerobic glycolysis was investigated using human biopsies. In hibernating myocardium showing an increase in anaerobic glycolytic flux metabolizing exogenous glucose, the adjustment of flux through this pathway was analyzed by flux:metabolite co-responses. By this means, a previously unknown pattern of regulation using multisite modulation was found which largely differs from traditional concepts of metabolic control of the Embden–Meyerhof pathway in normal and diseased myocardium.

【 授权许可】

Unknown