Wnt signaling related transcripts and their relationship to energy metabolism in C2C12 myoblasts under temperature stress

PeerJ

article

Marua Abu Risha¹  Asghar Ali¹  Puntita Siengdee¹  Nares Trakooljul¹  Fiete Haack¹  Dirk Dannenberger²  Klaus Wimmers³  Siriluck Ponsuksili¹ 

[1] Institute of Genome Biology, Functional Genome Analysis Research Unit, Leibniz Institute for Farm Animal Biology;Institute of Muscle Biology and Growth, Leibniz Institute for Farm Animal Biology;Institute of Genome Biology, Genomics Research Unit, Leibniz Institute for Farm Animal Biology;Faculty of Agriculture and Environmental Science, University Rostock

关键词: C2C12;    Energy metabolism;    Glycolysis;    Oxidative phosphorylation;    Heat/cold stress;    Wnt signalling;    Transcripts;   

DOI  :  10.7717/peerj.11625

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Temperature stress is one of the main environmental stressors affecting the welfare, health and productivity of livestock. Temperature changes can modify cell membrane components, disrupting the crosstalk between the cell and its surroundings by affecting signaling pathways including Wnt signaling pathway, which subsequently disrupts cell energy metabolism. The present study aims to understand the effect of temperature stress on the expression of genes involved in Wnt signaling pathways, and their interaction with energy metabolism in C2C12 myoblasts cells. The C2C12 cells were exposed to cold stress (35 °C), mild heat stress (39 °C) and severe heat stress (41 °C), whereas 37 °C was used as control temperature. Transcript levels of important genes involved in Wnt signaling including Axin2, Tnks2, Sfrp1, Dkk1, Dact1, Cby1, Wnt5a, Wnt7a, Wnt11, Porcn, Ror2, Daam1, and Ppp3ca were significantly altered under severe heat stress (41 °C), whereas eight Wnt signaling-related transcripts (Daam1, Ppp3ca, Fzd7, Wnt5a, Porcn, Tnks2, Lrp6, and Aes) were significantly altered under cold stress (35 °C) compared to control. Under heat stress transcripts of the Wnt/β-catenin inhibitors (Sfrp1, Dkk1, and Cby1) and negative regulators (Dact1 and Axin2) are activated. A positive correlation between oxidative phosphorylation and Wnt-related transcripts was found under high temperatures. Transcripts of the cell membrane receptors, including Lrp6 and Fzd7, and the members of Wnt/Ca+2 signaling pathway, including Ppp3ca and Porcn were downregulated under cold stress. Many Wnt signaling-related transcripts were positively correlated with glycolysis under cold stress. These findings indicate a cross-talk between Wnt signaling and energy metabolism under thermal stress.

【 授权许可】

CC BY