Proteomic analysis of the Plasmodium male gamete reveals the key role for glycolysis in flagellar motility

Malaria Journal

Research

Sanjeev Krishna¹  David R Graham²  Ceereena Ubaida-Mohien²  Andrea Ecker³  Mara Lawniczak³  Sara Marques³  Robert E Sinden³  Georges K Christophides³  Rebecca S Stanway⁴  Arthur M Talman⁵  Roland Frank⁶  Michael JE Sternberg⁷  Mark N Wass⁸  John R Yates⁹  Judith H Prieto^1,10  Tao Xu^1,11  Rhoel R Dinglasan^1,12 

[1] Centre for Infection, Division of Cellular and Molecular Medicine, St George’s, University of London, Cranmer Terrace, SW17 0RE, London, UK;Department of Molecular & Comparative Pathobiology, Johns Hopkins School of Medicine, 733 N Broadway, 21205, Baltimore, MD, USA;Division of Cell and Molecular Biology, Imperial College, London, UK;Division of Cell and Molecular Biology, Imperial College, London, UK;Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Str 74, 20359, Hamburg, Germany;Division of Cell and Molecular Biology, Imperial College, London, UK;Department of Microbial Pathogenesis, Yale University School of Medicine, 06510, New Haven, CT, USA;Research Centre for Infectious Diseases, University of Würzburg, Röntgenring 11, 97070, Würzburg, Germany;The Centre for Bioinformatics, Department of Life sciences, Imperial College, SW7 2AZ, London, UK;The Centre for Bioinformatics, Department of Life sciences, Imperial College, SW7 2AZ, London, UK;Centre for Molecular Processing, School of Biosciences, University of Kent, CT2 7NH, Canterbury, Kent, UK;The Scripps Research Institute, 92037, La Jolla, CA, USA;The Scripps Research Institute, 92037, La Jolla, CA, USA;Chemistry Department, Western Connecticut State University, Danbury, CT, USA;The Scripps Research Institute, 92037, La Jolla, CA, USA;Dow AgroSciences LLC, 9330 Zionsville Road, 46268, Indianapolis, IN, USA;W Harry Feinstone Department of Molecular Microbiology & Immunology, The Johns Hopkins Malaria Research Institute, 615 N Wolfe Street, 21205, Baltimore, MD, USA;

关键词: Gamete;    Plasmodium;    Glycolysis;    Flagellum;    Energy metabolism;   

DOI  :  10.1186/1475-2875-13-315

 received in 2014-06-11, accepted in 2014-07-28,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundGametogenesis and fertilization play crucial roles in malaria transmission. While male gametes are thought to be amongst the simplest eukaryotic cells and are proven targets of transmission blocking immunity, little is known about their molecular organization. For example, the pathway of energy metabolism that power motility, a feature that facilitates gamete encounter and fertilization, is unknown.MethodsPlasmodium berghei microgametes were purified and analysed by whole-cell proteomic analysis for the first time. Data are available via ProteomeXchange with identifier PXD001163.Results615 proteins were recovered, they included all male gamete proteins described thus far. Amongst them were the 11 enzymes of the glycolytic pathway. The hexose transporter was localized to the gamete plasma membrane and it was shown that microgamete motility can be suppressed effectively by inhibitors of this transporter and of the glycolytic pathway.ConclusionsThis study describes the first whole-cell proteomic analysis of the malaria male gamete. It identifies glycolysis as the likely exclusive source of energy for flagellar beat, and provides new insights in original features of Plasmodium flagellar organization.

【 授权许可】

CC BY   
© Talman et al.; licensee BioMed Central Ltd. 2014