Carbohydrate carriers affect adhesion of H. pylori to immobilized Leb‐oligosaccharide

FEBS Letters

Sato, Yoshia¹  Mizuochi, Tsuguo¹  Seino, Keita¹  Kojima, Naoya¹ 

[1] Department of Applied Biochemistry, and Institute of Glycotechnology, Tokai University, Hiratsuka, Kanagawa 259-1292, Japan

关键词: Lewis b;    Neoglycoprotein;    Neoglycolipid;    Bacterial adhesion;    Helicobacter pylori;    BSA;    bovine serum albumin;    DPPE;    dipalmitoylphosphatidylethanolamine;    PAA;    polyacrylamide;    Leb-BSA;    Leb-hexasaccharide-conjugated BSA;    Leb-DPPE;    neoglycolipid constructed with Leb-hexasaccharide and DPPE;    Leb-PAA;    Leb-tetrasaccharide-conjugated PAA;   

DOI  :  10.1016/S0014-5793(02)02571-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The present study involved comparison of adhesion of Helicobacter pylori KH202 to immobilized Le^b-oligosaccharide carried on different carriers, i.e. Le^b-oligosaccharide conjugated with polyacrylamide, bovine serum albumin, and dipalmitoylphosphatidylethanolamine (Le^b-PAA, Le^b-BSA, and Le^b-DPPE). All of the Le^b-oligosaccharide-carrying neoglycoconjugates served as ligands for H. pylori. However, H. pylori required 10-fold and 100-fold quantities of Le^b-antigen to adhere to Le^b-PAA and to Le^b-DPPE in comparison to the quantity of Le^b-antigen needed to adhere to Le^b-BSA, respectively. H. pylori adhesion to Le^b-PAA and Le^b-DPPE was clearly inhibited by Le^b-oligosaccharide, but adhesion to Le^b-BSA was hardly inhibited by the oligosaccharide. Therefore, the carbohydrate carrier affects the affinity of H. pylori KH202 toward Le^b-antigen, although the bacteria recognize Le^b-antigen regardless of the carbohydrate carrier.

【 授权许可】

Unknown