Requirement of ceramide for adhesion of Helicobacter pylori to glycosphingolipids

FEBS Letters

Tang, Wei³  Ito, Mieko³  Makuuchi, Masatoshi¹  Mizuochi, Tsuguo³  Seino, Keita³  Senda, Hisato⁴  Kojima, Naoya³  Konishi, Toshiro² 

[1] Department of Surgery, Graduate School of Medicine, University of Tokyo, Bunkyo, Tokyo 113-8655, Japan;Department of Surgery, Kanto Medical Center, NTT EC, Shinagawa, Tokyo 141-8625, Japan;Department of Applied Biochemistry, Tokai University, Hiratsuka, Kanagawa 259-1292, Japan;Central Research Laboratories, Kaken Pharmaceutical Co., Ltd., Yamashina, Kyoto 607-8042, Japan

关键词: Glycosphingolipid;    Neoglycolipid;    Neoglycoprotein;    Neoglycoconjugate;    Bacterial adhesion;    Helicobacter pylori;    BSA;    bovine serum albumin;    DPPE;    dipalmitoylphosphatidylethanolamine;    PAA;    polyacrylamide;    RCA;    Ricinus communis agglutinin;    GSL;    glycosphingolipid;   

DOI  :  10.1016/S0014-5793(01)02759-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Direct adhesion of Helicobacter pylori to immobilized glycosphingolipids (GSLs) was compared to that of their corresponding oligosaccharide-conjugated neoglycoconjugates in order to clarify the roles of the carbohydrate and lipid portions of GSLs in H. pylori adhesion. These bacteria were found to adhere to sulfatide, GM3, GalCer and LacCer, but not to ceramide, sphingomyelin, or polyacrylamides conjugated with β-galactose, lactose, 3′-sialyllactose or 3′-sulfo-β-galactose. Furthermore, neoglycolipids or bovine serum albumin derivatives with corresponding oligosaccharides were unable to serve as the ligands. H. pylori adhesion to GalCer with α-hydroxyl fatty acid was much stronger than GalCer with the non-hydroxyl fatty acid. These results suggest that H. pylori recognize the conformation of GSL with α-hydroxyl fatty acid on solid phase.

【 授权许可】

Unknown