Differential expression of β1,3galactosyltransferases in human colon cells derived from adenocarcinomas or normal mucosa 1

FEBS Letters

Valli, Maurizia¹  Trinchera, Marco¹  Bardoni, Anna¹ 

[1] Department of Biochemistry, University of Pavia, via Taramelli 3B, 27100 Pavia, Italy

关键词: Galactosyltransferase;    Type 1 chain;    Sialyl-Lewis a antigen;    Carcinoembryonic antigen;    Colon adenocarcinoma;    Competitive PCR;    Lea;    Lewis a;    Galβ1-3[Fucα1-4]GlcNAc;    sialyl-Lea;    sialyl-Lewis a;    NeuAcα2-3Galβ1-3[Fucα1-4]GlcNAc;    Leb;    Lewis b;    Fucα1-2Galβ1-3[Fucα1-4]GlcNAc;    CEA;    carcinoembryonic antigen;    NCA-2;    non-specific cross-reacting antigen 2;    NFA-2;    normal fecal antigen 2;    FUT2;    secretor type α1;    2fucosyltransferase;    Fuc-TIII;    α1;    3/1;    4fucosyltransferase;    GalT;    galactosyltransferase;    RT-PCR;    reverse transcriptase mediated-PCR;   

DOI  :  10.1016/S0014-5793(99)00547-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two β1,3galactosyltransferases are detected in human colon cells: one corresponds to β3GalT1, the other (β3GalTx) is found to be different from any cloned β3GalT since in vitro it utilizes GlcNAc very efficiently under specific reaction conditions. Expression of β3GalT1 transcript is high in normal colon mucosa and control neuroectodermal cells, which do not express sialyl-Lewis a antigen, and low in colon adenocarcinoma cells, as assessed by competitive RT-PCR. β3GalTx activity is high in adenocarcinoma cells expressing sialyl-Lewis a and undetectable in all other cells, suggesting differential involvement and opposite regulation of such enzymes during carcinogenesis.

【 授权许可】

Unknown