FEBS Letters | |
Targeted cytosolic delivery of hydrogel nanoparticles into HepG2 cells through engineered Sendai viral envelopes | |
Gupta, Chhitar M1  Jana, Siddhartha S3  Maitra, Amarnath2  Sarkar, Debi P3  Mani, Prashant3  Bharali, Dhruba J2  | |
[1] Division of Molecular and Structural Biology, Central Drug Research Institute, P.O. Box 173, Lucknow 226001, India;Department of Chemistry, University of Delhi, Delhi 110007, India;Department of Biochemistry, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India | |
关键词: Hydrogel nanoparticle; Sendai virus; Virosome; Drug delivery; Targeted cytosolic delivery; Controlled release; ASGP-R; asialoglycoprotein receptor; CHO; Chinese hamster ovary; DMEM; Dulbecco's modified Eagle's medium; DPBS; Dulbecco's phosphate-buffered saline; EDTA; ethylenediaminetetraacetate (disodium salt); F; fusion protein; FCS; fetal calf serum; FITC-Dx; fluorescein isothiocyanate dextran (mol wt. 19.3 kDa); PL; polylysine; PVPf-NP; FITC-Dx-loaded polyvinylpyrrolidone nanoparticle; TBS; Tris-buffered saline; | |
DOI : 10.1016/S0014-5793(02)02467-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Hydrogel nanoparticles of cross-linked polyvinylpyrrolidone (PVP-NP) (35–50 nm in diameter) containing fluoresceinated dextran (FITC-Dx) were encapsulated in reconstituted Sendai viral envelopes containing only the fusion (F) protein (F-virosomes1). Incubation of these loaded F-virosomes with human hepatoblastoma cells (HepG2) in culture resulted in membrane-fusion-mediated delivery of NPs to the cell cytoplasm, as inferred from the ability of cells to internalize FITC-Dx loaded PVP-NP (PVPf-NP) in the presence of azide (an inhibitor of the endocytotic process). Introduction of PVPf-NP into the HepG2 cells was assured by selective accumulation of FITC fluorescence in the cytosolic compartment. The structural integrity of the internalized PVPf-NP was also confirmed by fluorescence microscopy and ultracentrifugation analysis. The potential usefulness of PVP-NP-mediated cytosolic release of water soluble drugs both in vitro and in vivo has been established for the first time.
【 授权许可】
Unknown
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