FEBS Letters | |
F protein induced fusion of Sendai viral envelopes with mouse teratocarcinoma cells through LeX‐LeX interaction | |
Kumar, Mukesh1  Sarkar, Debi P.1  | |
[1] Department of Biochemistry, University of Delhi South Campus, Benito Juarez Road, New Delhi - 110021, India | |
关键词: Sendai; Virosome; Lex/CD15; Membrane fusion; F; fusion factor; CHO; Chinese hamster ovary; DMEM; Dulbecco's modified Eagle's medium; DPBS; Dulbecco's phosphate-buffered saline; TX-100; Triton X-100; DTT; dithiothreitol; NBD-PE; N-4-nitrobenzo-2-oxa-1; 3-diazole phosphatidylethanolamine; FCS; fetal calf serum; | |
DOI : 10.1016/0014-5793(96)00698-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The efficiency of membrane fusion between reconstituted Sendau viral envelopes containing only the fusion protein (F-virosomes) and the plasma membrane of mouse teratocarcinoma cells (F9) in culture was assessed using an assay based on the relief of self-quenching of a lipid probe incorporated in the F-virosomes. The potential of F-virosomes was also evaluated for a targeted cytosolic delivery of lysozyme to F9 cells. [125I]Lysozyme entrapped into F-virosomes was taken to examine its fusion-mediated transfer to the F9 cells. Target specificity of the F-virosomes was confirmed by the interaction between the terminal Lex moiety (Ga1β1 → 4(Fucα1 → 3)GlcNac) of F protein and the Lex determinant on the membrane of F9 cells. Incubation of the loaded F-virosomes with cells led to fusion-mediated delivery, as inferred from the ability of cells to internalize lysozyme in the presence of azide (a potent inhibitor of endocytosis). These results suggest that carbohydrate-carbohydrate interaction is strong enough for target cell recognition followed by phospholipid bilayer melding induced by fusion glycoprotein of Sendai virus.
【 授权许可】
Unknown
【 预 览 】
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