Fusion of reconstituted influenza virus envelopes with liposomes mediated by streptavidin/biotin interactions

FEBS Letters

Wilschut, Jan²  Schoen, Pieter²  Leserman, Lee¹ 

[1] Centre d'Immunologie, Institut National de la Santé et de la Recherche Médicale-Centre National de la Recherche Scientifique de Marseille-Luminy, Case 906, 13288 Marseille Cedex 9, France;Groningen Utrecht Institute for Drug Exploration (GUIDE), Department of Physiological Chemistry, University of Groningen, A. Deusinglaan 1, 9713 AV Groningen, The Netherlands

关键词: Influenza virus;    Hemagglutinin;    Virosome;    Membrane fusion;    Targeting;    Cellular delivery;    Carrier system;    HA;    hemagglutinin;    PE;    phosphatidylethanolamine;    biotinPE;    biotin-derivatized PE;    PC;    phosphatidylcholine;    pyrPC;    pyrene-labeled PC;    C12E8;    octaetheleneglycol monododecyl ether;    HBS;    HEPES-buffered saline;    DOPC;    dioleoylPC;    DOPE;    dioleoylPE;    BSA;    bovine serum albumin;    R18;    octadecylrhodamine;   

DOI  :  10.1016/0014-5793(96)00682-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Reconstituted influenza virus envelopes (virosomes) containing the viral hemagglutinin (HA) represent an efficient fusogenic cellular delivery system. By interaction of HA with its natural receptors, sialylated lipids (gangliosides) or proteins, virosomes bind to cells and, following endocytic uptake, deliver their contents to the cytosol through fusion from within acidic endosomes. Here, we show that binding to sialic acid is not necessary for fusion. In the presence of streptavidin, virosomes containing a biotinylated lipid fused with liposomes lacking sialic acid if these liposomes also had a biotinylated lipid in their membranes. Moreover, fusion characteristics corresponded well with fusion of virosomes with ganglioside-containing liposomes.

【 授权许可】

Unknown