FEBS Letters | |
Influenza hemagglutinin mediated fusion of membranes containing poly(ethylene‐glycol) grafted lipids: new insights into the fusion mechanism | |
Bonnafous, Pierre1  Stegmann, Toon1  Chams, Vida1  | |
[1] Institut de Pharmacologie et de Biologie Structurale, CNRS UPR 9062, 205 Route de Narbonne, 31077 Toulouse, France | |
关键词: Influenza virus; Membrane fusion; Poly(ethylene-glycol); Hemagglutinin; | |
DOI : 10.1016/S0014-5793(99)00333-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The influence of a hydrophilic layer covering the membrane on influenza hemagglutinin (HA) mediated fusion was investigated using membranes containing poly(ethylene-glycol) grafted phosphatidylethanolamine (PEG-2000-PE). Steric inhibition of HA-membrane interactions by these lipids affected virus fusion (half-maximal inhibition at 0.8 mol% for lipids with 114 ethylene glycol residues, or at 3.2 mol% for 45 residues (PEG-2000-PE), concentrations at which the PEG moieties adopt a random coil structure). Reconstituted viral membranes containing 3 mol% PEG-2000-PE retained 40% of their fusion activity. Therefore, efficient fusion is possible with membranes completely covered by a hydrophilic layer of several nanometers, and fusogenic virosomes containing PEG-PE are feasible.
【 授权许可】
Unknown
【 预 览 】
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RO201912020307501ZK.pdf | 129KB | download |