Influenza hemagglutinin mediated fusion of membranes containing poly(ethylene‐glycol) grafted lipids: new insights into the fusion mechanism

FEBS Letters

Bonnafous, Pierre¹  Stegmann, Toon¹  Chams, Vida¹ 

[1] Institut de Pharmacologie et de Biologie Structurale, CNRS UPR 9062, 205 Route de Narbonne, 31077 Toulouse, France

关键词: Influenza virus;    Membrane fusion;    Poly(ethylene-glycol);    Hemagglutinin;   

DOI  :  10.1016/S0014-5793(99)00333-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The influence of a hydrophilic layer covering the membrane on influenza hemagglutinin (HA) mediated fusion was investigated using membranes containing poly(ethylene-glycol) grafted phosphatidylethanolamine (PEG-2000-PE). Steric inhibition of HA-membrane interactions by these lipids affected virus fusion (half-maximal inhibition at 0.8 mol% for lipids with 114 ethylene glycol residues, or at 3.2 mol% for 45 residues (PEG-2000-PE), concentrations at which the PEG moieties adopt a random coil structure). Reconstituted viral membranes containing 3 mol% PEG-2000-PE retained 40% of their fusion activity. Therefore, efficient fusion is possible with membranes completely covered by a hydrophilic layer of several nanometers, and fusogenic virosomes containing PEG-PE are feasible.

【 授权许可】

Unknown