Crystal structure of plant pectin methylesterase

FEBS Letters

Eklund, Hans²  Johansson, Kenth²  El-Ahmad, Mustapha²  Markovič, Oskar³  Friemann, Rosmarie²  Jörnvall, Hans¹ 

[1] Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S-171 77 Stockholm, Sweden;Department of Molecular Biology, Swedish University of Agricultural Sciences, S-751 24 Uppsala, Sweden;Institute of Chemistry, Slovak Academy of Sciences, 842 38 Bratislava, Slovak Republic

关键词: Crystal structure;    β-Helix;    Pectin methylesterase;    Plant enzyme;    Catalytic mechanism;    Binding cleft;   

DOI  :  10.1016/S0014-5793(02)02372-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pectin is a principal component in the primary cell wall of plants. During cell development, pectin is modified by pectin methylesterases to give different properties to the cell wall. This report describes the first crystal structure of a plant pectin methylesterase. The β-helical structure embodies a central cleft, lined by several aromatic residues, that has been deduced to be suitable for pectin binding. The active site is found at the center of this cleft where Asp157 is suggested to act as the nucleophile, Asp136 as an acid/base and Gln113/Gln135 to form an anion hole to stabilize the transition state.

【 授权许可】

Unknown