Two Arabidopsis thaliana genes encode functional pectin methylesterase inhibitors1

FEBS Letters

Camardella, L²  Cervone, F³  Raiola, A³  Giovane, A¹  Mattei, B³  De Lorenzo, G³  Bellincampi, D³ 

[1] Department of Biochemistry and Biophysics, 2nd University of Naples, Via Costantinopoli 16, I-80138 Napoli, Italy;Institute of Protein Biochemistry, CNR, Via Marconi 10, I-80125 Napoli, Italy;Dipartimento di Biologia Vegetale, Laboratorio di Genomica Funzionale e Proteomica, Università di Roma ‘La Sapienza’, Piazzale Aldo Moro 5, 00185 Roma, Italy

关键词: Plant cell wall;    Pectin;    Pectin methylesterase;    Pectin methylesterase inhibitor;    Arabidopsis thaliana;    PME;    pectin methylesterase;    PMEI;    pectin methylesterase inhibitor;    SPR;    surface plasmon resonance;   

DOI  :  10.1016/S0014-5793(03)01491-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have identified, expressed and characterized two genes from Arabidopsis thaliana (AtPMEI-1 and AtPMEI-2) encoding functional inhibitors of pectin methylesterases. AtPMEI-1 and AtPMEI-2 are cell wall proteins sharing many features with the only pectin methylesterase inhibitor (PMEI) characterized so far from kiwi fruit. Both Arabidopsis proteins interact with and inhibit plant-derived pectin methylesterases (PMEs) but not microbial enzymes. The occurrence of functional PMEIs in Arabidopsis indicates that a mechanism of controlling pectin esterification by inhibition of endogenous PMEs is present in different plant species.

【 授权许可】

Unknown