Novel approach reveals localisation and assembly pathway of the PsbS and PsbW proteins into the photosystem II dimer

FEBS Letters

Robinson, Colin²  P. Schröder, Wolfgang³  Thidholm, Ellinor¹  Funk, Christiane¹  Tissier, Christophe²  Lindström, Viktoria¹ 

[1]Arrhenius Laboratories for Natural Sciences, Department of Biochemistry and Biophysics, Stockholm University, SE-106 91 Stockholm, Sweden

[2]Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK

[3]Södertörns Högskola (University College), Bipontus, P.O. Box 4101, SE-141 04 Huddinge, Sweden

关键词: Assembly;    PsbS;    PsbW;    Photosystem II dimer;    Import;    Blue-native polyacrylamide gel electrophoresis;    BN-PAGE;    blue-native polyacrylamide gel electrophoresis;    β-DM;    n-dodecyl-β-D-maltoside;    LHCII;    light harvesting complex II;    pPsbW;    pPsbS;    precursors of PsbW and PsbS;    respectively;    PSI;    PSII;    photosystem I and photosystem II;    respectively;    PVDF;    polyvinylidene difluoride;    Rubisco;    ribulose-1;    5-bisphosphate carboxylase/oxygenase;    SDS;    sodium dodecyl sulphate;   

DOI  :  10.1016/S0014-5793(02)02314-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A blue-native gel electrophoresis system was combined with an in organello import assay to specifically analyse the location and assembly of two nuclear-encoded photosystem II (PSII) subunits. With this method we were able to show that initially the low molecular mass PsbW protein is not associated with the monomeric form of PSII. Instead a proportion of newly imported PsbW is directly assembled in dimeric PSII supercomplexes with very fast kinetics; its negatively charged N-terminal domain is essential for this process. The chlorophyll-binding PsbS protein, which is involved in energy dissipation, is first detected in the monomeric PSII subcomplexes, and only at later time points in the dimeric form of PSII. It seems to be bound tighter to the PSII core complex than to light harvesting complex II. These data point to radically different assembly pathways for different PSII subunits.

【 授权许可】

Unknown