Production of superoxide from Photosystem II in a rice (Oryza sativaL.) mutant lacking PsbS

BMC Plant Biology

Research Article

Gynheung An¹  Ung Chan Yoon²  Mainak Banerjee²  Choon-Hwan Lee³  Yong-Hwan Moon³  Krishna Nath³  Young-Jae Eu³  Bolormaa Dogsom³  Roshan Sharma Poudyal³  Altanzaya Tovuu⁴  Ismayil S Zulfugarov⁵  Michael Hall⁶  Stefan Jansson⁶ 

[1] Crop Biotech Institute, Kyung Hee University, 446-701, Yongin, Korea;Department of Chemistry, Pusan National University, Jangjeon-dong, 609-735, Keumjung-gu, Busan, Korea;Department of Integrated Biological Science and Department of Molecular Biology, Pusan National University, 609-735, Busan, Korea;Department of Integrated Biological Science and Department of Molecular Biology, Pusan National University, 609-735, Busan, Korea;Department of Biology, Mongolian State University of Agriculture, Zaisan, 17024, Ulaanbaatar, Mongolia;Department of Integrated Biological Science and Department of Molecular Biology, Pusan National University, 609-735, Busan, Korea;Department of Biology, North-Eastern Federal University, 58 Belinsky Str, 677-027, Yakutsk, Republic of Sakha (Yakutia), Russian Federation;Institute of Botany, Azerbaijan National Academy of Sciences, Patamdar Shosse 40, 1073, Baku, AZ, Azerbaijan;Umeå Plant Science Center, Department of Plant Physiology, Umeå University, SE-901 87, Umeå, Sweden;

关键词: Photoprotection;    PsbS;    ROS;    Superoxide;    Photosynthesis;    NPQ;    Rice;   

DOI  :  10.1186/s12870-014-0242-2

 received in 2014-04-24, accepted in 2014-09-08,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundPsbS is a 22-kDa Photosystem (PS) II protein involved in non-photochemical quenching (NPQ) of chlorophyll fluorescence. Rice (Oryza sativa L.) has two PsbS genes, PsbS1 and PsbS2. However, only inactivation of PsbS1, through a knockout (PsbS1-KO) or in RNAi transgenic plants, results in plants deficient in qE, the energy-dependent component of NPQ.ResultsIn studies presented here, under fluctuating high light, growth of young seedlings lacking PsbS is retarded, and PSII in detached leaves of the mutants is more sensitive to photoinhibitory illumination compared with the wild type. Using both histochemical and fluorescent probes, we determined the levels of reactive oxygen species, including singlet oxygen, superoxide, and hydrogen peroxide, in leaves and thylakoids. The PsbS-deficient plants generated more superoxide and hydrogen peroxide in their chloroplasts. PSII complexes isolated from them produced more superoxide compared with the wild type, and PSII-driven superoxide production was higher in the mutants. However, we could not observe such differences either in isolated PSI complexes or through PSI-driven electron transport. Time-course experiments using isolated thylakoids showed that superoxide production was the initial event, and that production of hydrogen peroxide proceeded from that.ConclusionThese results indicate that at least some of the photoprotection provided by PsbS and qE is mediated by preventing production of superoxide released from PSII under conditions of excess excitation energy.

【 授权许可】

Unknown   
© Zulfugarov et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.