Curcumin induces apoptosis in human breast cancer cells through p53‐dependent Bax induction

FEBS Letters

Sa, Gaurisankar²  Choudhuri, Tathagata²  Agwarwal, Munna L¹  Das, Tanya²  Pal, Suman² 

[1]Department of Molecular Biology, The Learner Research Institute, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA

[2]Animal Physiology Section, Bose Institute, P-1/12 CIT Scheme VII M, Kolkata 700 054, India

关键词: Apoptosis;    Breast cancer;    Curcumin;    p53;    Bax;    Bcl-2;    DAPI;    4′;    6′-diamidino-2-phenylindole;   

DOI  :  10.1016/S0014-5793(02)02292-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The aim of this study was to determine the mechanisms of curcumin-induced human breast cancer cell apoptosis. From quantitative image analysis data showing an increase in the percentage of cells with a sub-G0/G1 DNA content, we demonstrated curcumin-induced apoptosis in the breast cancer cell line MCF-7, in which expression of wild-type p53 could be induced. Apoptosis was accompanied by an increase in p53 level as well as its DNA-binding activity followed by Bax expression at the protein level. Further experiments using p53-null MDAH041 cell as well as low and high p53-expressing TR9-7 cell, in which p53 expression is under tight control of tetracycline, established that curcumin induced apoptosis in tumor cells via a p53-dependent pathway in which Bax is the downstream effector of p53. This property of curcumin suggests that this molecule could have a possible therapeutic potential in breast cancer patients.

【 授权许可】

Unknown