FEBS Letters | |
Curcumin induces apoptosis in human breast cancer cells through p53‐dependent Bax induction | |
Sa, Gaurisankar2  Choudhuri, Tathagata2  Agwarwal, Munna L1  Das, Tanya2  Pal, Suman2  | |
[1] Department of Molecular Biology, The Learner Research Institute, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA;Animal Physiology Section, Bose Institute, P-1/12 CIT Scheme VII M, Kolkata 700 054, India | |
关键词: Apoptosis; Breast cancer; Curcumin; p53; Bax; Bcl-2; DAPI; 4′; 6′-diamidino-2-phenylindole; | |
DOI : 10.1016/S0014-5793(02)02292-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The aim of this study was to determine the mechanisms of curcumin-induced human breast cancer cell apoptosis. From quantitative image analysis data showing an increase in the percentage of cells with a sub-G0/G1 DNA content, we demonstrated curcumin-induced apoptosis in the breast cancer cell line MCF-7, in which expression of wild-type p53 could be induced. Apoptosis was accompanied by an increase in p53 level as well as its DNA-binding activity followed by Bax expression at the protein level. Further experiments using p53-null MDAH041 cell as well as low and high p53-expressing TR9-7 cell, in which p53 expression is under tight control of tetracycline, established that curcumin induced apoptosis in tumor cells via a p53-dependent pathway in which Bax is the downstream effector of p53. This property of curcumin suggests that this molecule could have a possible therapeutic potential in breast cancer patients.
【 授权许可】
Unknown
【 预 览 】
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