Participation of the mitochondrial permeability transition pore in nitric oxide‐induced plant cell death

FEBS Letters

Salgado, Ione¹  Orsi, Cintia H.¹  Oliveira, Jusceley F.P.¹  Pinto-Maglio, Cecı́lia A.F.²  Saviani, Elzira E.¹ 

[1] Departamento de Bioquı́mica, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), CP 6109, Campinas, SP, CEP 13083-970, Brazil;Centro de Genética, Biologia Molecular e Fitoquı́mica, Instituto Agronômico de Campinas (IAC), CP 28, Campinas, SP, CEP 13001-970, Brazil

关键词: Nitric oxide;    Programmed cell death;    Mitochondrial permeability transition pore;    Apoptosis;    Citrus cell;    CMXRos;    chloromethyl X-rosamine;    CsA;    cyclosporin A;    CYSNO;    nitrosocysteine;    FCCP;    carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone;    GSNO;    S-nitrosoglutathione;    ΔΨ m;    membrane electrical potential;    PBS;    phosphate-buffered saline;    PCD;    programmed cell death;    PTP;    permeability transition pore;    SNAP;    S-nitroso-N-acetylpenicillamine;    SNP;    sodium nitroprusside;   

DOI  :  10.1016/S0014-5793(01)03230-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the present study, we investigated the involvement of the mitochondrial permeability transition pore (PTP) in nitric oxide (NO)-induced plant cell death. NO donors such as sodium nitroprusside (SNP) and S-nitroso-N-acetylpenicillamine inhibited growth and caused death in suspension-cultured cells of Citrus sinensis. Cells treated with SNP showed chromatin condensation and fragmentation, characteristic of apoptosis. SNP caused loss of the mitochondrial membrane electrical potential, which was prevented by cyclosporin A (CsA), a specific inhibitor of PTP formation. CsA also prevented the nuclear apoptosis and subsequent Citrus cell death induced by NO. These findings indicate that mitochondrial PTP formation is involved in the signaling pathway by which NO induces apoptosis in cultured Citrus cells.

【 授权许可】

Unknown