Plants expressing murine pro-apoptotic protein Bid do not have enhanced PCD

BMC Research Notes

Massimo Crimi¹  Anna Manara¹  Zahra Imanifard¹  Linda Fracasso¹  Diana Bellin¹ 

[1] Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy;

关键词: Programmed cell death;    Apoptosis;    BH3-only protein;    Bid;    Oxidative stress;    Nitric oxide;    Plant pathogen;   

DOI  :  10.1186/s13104-020-05285-x

来源: Springer

【 摘 要 】

ObjectivesThe purpose of this study was to explore whether plant programmed cell death (PCD) cascade can sense the presence of the animal-only BH3 protein Bid, a BCL-2 family protein known to play a regulatory role in the signaling cascade of animal apoptosis.ResultsWe have expressed the mouse pro-apoptotic protein Bid in Arabidopsis thaliana and in Nicotiana tabacum. We did not obtain any transformed plant constitutively expressing the truncated protein (tBid—i.e. the caspase-activated form) whereas ectopic expression of the full-length protein (flBid) does not interfere with growth and development of the transformed plants. To verify whether the presence of this animal pro-apoptotic protein modified stress responses and PCD execution, both N. tabacum and A. thaliana plants constitutively expressing flBid have been studied under different stress conditions triggering cell death activation. The results show that the presence of flBid in transgenic plants did not significantly change the responses to abiotic stress (H2O2 or NO) and biotic stress treatments. Moreover, the finding that no Bid active form was present in treated tobacco plants suggests an absence of a proper activation of Bid.

【 授权许可】

CC BY