Phosphatidylglycerophosphate synthases from Arabidopsis thaliana

FEBS Letters

Müller, Frank¹  Frentzen, Margrit¹ 

[1] RWTH Aachen, Institut für Biologie I, Spezielle Botanik, Worringer Weg 1, 52056 Aachen, Germany

关键词: CDP-diacylglycerol;    Functional expression;    Membrane lipid synthesis;    Phosphatidyltransferase;    Phosphatidylglycerophosphate synthase;    Arabidopsis thaliana;    PG;    phosphatidylglycerol;    PGP;    phosphatidylglycerophosphate;    PGPS;    phosphatidylglycerophosphate synthase;   

DOI  :  10.1016/S0014-5793(01)03163-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two Arabidopsis thaliana genes were shown to encode phosphatidylglycerophosphate synthases (PGPS) of 25.4 and 32.2 kDa, respectively. Apart from their N-terminal regions, the two proteins exhibit high sequence similarity. Functional expression studies in yeast provided evidence that the 25.4 kDa protein is a microsomal PGPS while the 32.2 kDa protein represents a preprotein which can be imported into yeast mitochondria and processed to a mature PGPS. The two isozymes were solubilized and purified as fusion proteins carrying a His tag at their C-terminus. Enzyme assays with both membrane fractions and purified enzyme fractions revealed that the two A. thaliana isozymes have similar properties but differ in their CDP-diacylglycerol species specificity.

【 授权许可】

Unknown