FEBS Letters | |
Phosphatidylglycerophosphate synthases from Arabidopsis thaliana | |
Müller, Frank1  Frentzen, Margrit1  | |
[1] RWTH Aachen, Institut für Biologie I, Spezielle Botanik, Worringer Weg 1, 52056 Aachen, Germany | |
关键词: CDP-diacylglycerol; Functional expression; Membrane lipid synthesis; Phosphatidyltransferase; Phosphatidylglycerophosphate synthase; Arabidopsis thaliana; PG; phosphatidylglycerol; PGP; phosphatidylglycerophosphate; PGPS; phosphatidylglycerophosphate synthase; | |
DOI : 10.1016/S0014-5793(01)03163-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Two Arabidopsis thaliana genes were shown to encode phosphatidylglycerophosphate synthases (PGPS) of 25.4 and 32.2 kDa, respectively. Apart from their N-terminal regions, the two proteins exhibit high sequence similarity. Functional expression studies in yeast provided evidence that the 25.4 kDa protein is a microsomal PGPS while the 32.2 kDa protein represents a preprotein which can be imported into yeast mitochondria and processed to a mature PGPS. The two isozymes were solubilized and purified as fusion proteins carrying a His tag at their C-terminus. Enzyme assays with both membrane fractions and purified enzyme fractions revealed that the two A. thaliana isozymes have similar properties but differ in their CDP-diacylglycerol species specificity.
【 授权许可】
Unknown
【 预 览 】
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RO201912020311279ZK.pdf | 227KB | download |