Characterization of reactions catalysed by yeast phosphatidylinositol synthase

FEBS Letters

Brandenburger, Yves¹  Klezovitch, Olga¹  Geindre, Michèle¹  Deshusses, Jacques¹ 

[1] Department of Biochemistry, University of Geneva, 30, Quai E.-Ansermet, 1211 Geneva 4, Switzerland

关键词: CDP-diacylglycerol;    Phosphatidylinositol synthase;    Exchange reaction;    Saccharomyces cerevisiae;    Ins;    inositol;    PI;    phosphatidylinositol;    CDPdG;    cytidine diphosphate diacylglycerol;    DOC-Na;    Sodium deoxycholate;    DTT;    dithiothreitol;   

DOI  :  10.1016/0014-5793(93)80598-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The nature of reactions catalysed by yeast phosphatidylinositol synthase expressed in E. coli has been investigated. The single enzyme is shown to carry both CDP-diacylglycerol-dependent incorporation of inositol into phosphatidylinositol (K _m for inositol of 0.090 mM) and a CDP-diacylglycerol-independent exchange reaction between phosphatidylinositol and inositol (K _m for inositol of 0.066 mM). The exchange reaction and reversal of phosphatidylinositol synthase were both stimulated by CMP, but had different optimum pH and requirements for substrates. These results suggest that CMP-stimulated exchange and CMP-dependent reverse reactions are distinct processes catalysed by the same enzyme. phosphatidylinositol synthase.

【 授权许可】

Unknown