The enhanced 32P labeling of CDP‐diacylglycerol in c‐myc gene expressed human kidney cancer cells

FEBS Letters

Inoue, Hiroko³  Yao, Masahiro²  Kubota, Yoshinobu²  Shuin, Taro²  Yoshioka, Tohru¹ 

[1] Department of Physiology, School of Medicine, Yokohama City University, 2-33, Urafune-cho, Minami-ku, Yokohama 232, Japan;Department of Urology, School of Medicine, Yokohama City University, 3-46, Urafune-cho, Minami-ku, Yokohama 232, Japan;School of Human Sciences, Waseda University, 1-6-1, Nishiwaseda, Shinjuku-ku, Tokyo 160 Japan

关键词: c-myc gene;    Phosphatidylinositol metabolism;    CDP-diacylglycerol;    (Kidney cancer cell);    PI;    phosphatidylinositol;    PIP;    phosphatidylinositol 4-phosphate;    PIP2;    phosphatidylinositol 4;    5-bisphosphate;    PG;    phosphatidylglycerol;    TLC;    thin-layer chromatography;    HPTLC;    high-performance TLC;   

DOI  :  10.1016/0014-5793(87)81577-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Correlation between c-myc gene expression and phosphatidylinositol (PI) metabolism was studied using seven human kidney cancer cell lines. We found that the exceptional incorporation of ³²P into CDP-diacyl-glycerol (CDP-DG) was observed in only two cell lines, in which c-myc expression was increased among seven cell lines tested. In these two cell lines, PI was also labeled. The other five cell lines indicated the accumulation of radioactive PI, whereas CDP- DG was unlabeled.

【 授权许可】

Unknown