RNA ligands generated against complex nuclear targets indicate a role for U1 snRNP in co‐ordinating transcription and RNA splicing

FEBS Letters

Tian, Huicheng¹ 

[1]Department of Genetics, Center for Genetic and Cellular Therapies, Duke University Medical Center, Durham, NC 27710, USA

关键词: RNA ligand;    U1 small nuclear ribonucleoprotein particle;    RNA polymerase II;    Transcription;    Splicing;    Pol II;    RNA polymerase II;    CTD;    carboxyl-terminal domain;    snRNP;    small nuclear ribonucleoprotein particles;    SELEX;    systematic evolution of ligands by exponential enrichment;    RT/PCR;    reverse transcription/polymerase chain reaction;    nt;    nucleotide(s);    ds;    double-stranded;    CMV;    cytomegalovirus;   

DOI  :  10.1016/S0014-5793(01)03188-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

RNA ligands were generated against various gene products present in HeLa nuclear extract. Functional profiling was performed to identify RNA ligands that modulate RNA polymerase II (pol II)-mediated transcription. Unexpectedly, four of the eight inhibitor ligands identified by this screen contained an 11-nucleotide sequence identical to the 5′-splice site of eukaryotic pre-mRNAs. Such ligands were shown to impede pre-initiation complex assembly on a cytomegalovirus promoter. In addition, U1 small nuclear ribonucleoprotein particles (snRNP) and pol II had been co-immunoprecipitated in the absence of transcription. These results suggest a role for U1 snRNP in co-ordinating transcription and RNA splicing.

【 授权许可】

Unknown