Paternally inherited transgenes are down‐regulated but retain low activity during early embryogenesis in Arabidopsis

FEBS Letters

Baroux, Célia¹  Blanvillain, Robert¹  Gallois, Patrick¹ 

[1] Génome et Développement des Plantes, Université de Perpignan, 52 avenue de Villeneuve, 66860 Perpignan Cedex, France

关键词: Paternal gene expression;    Embryo development;    BARNASE;    Transactivation;    Arabidopsis;    DAP;    days after pollination;    GUS;    β-glucuronidase;    wt;    wild-type;   

DOI  :  10.1016/S0014-5793(01)03097-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated the timing of transgene activation after fertilisation in Arabidopsis following crosses and using two transgenic promoters (from the AtCYCB1 and AtLTP1 genes). Using both a transactivation system and direct transcriptional fusion to drive β-glucuronidase reporter expression, reciprocal crosses showed a lack of expression of the paternal components. This is consistent with a lack of paternal genome activity previously reported during early seed development in Arabidopsis [Viella-Calzada et al. (2000) Nature 404, 91–94]. However, transactivation experiments of the BARNASE gene gave evidence that at least some paternal loci retain transcriptional activity, though at a low level, during early embryogenesis.

【 授权许可】

Unknown