Combined assessment of cyto-, geno-, and embryotoxicity of silica nanoparticles in experiments in vitro

5th Nanosafe International Conference on Health and Safety issues related to nanomaterials for a socially responsible approach

物理学;医药卫生;材料科学

Protasova, G.A.^1 ; Popov, V.B.^1 ; Shabasheva, L.V.^1 ; Strekalovskii, I.V.^1 ; Panferova, Y.A.^1 ; Khlebnikova, N.S.^1 ; Radilov, A.A.^1

Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency, G/P Kuzmolovsky, Vsevolozhsky District, Leningrad Region

199663, Russia^1

关键词: Chromosomal aberration;    Embryo development;    Embryotoxicity;    Human peripheral blood;    Mutagenicity;    Nanoparticle (NPs);    Post-implantation;    Simultaneous use;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/838/1/012029/pdf
DOI  :  10.1088/1742-6596/838/1/012029

来源: IOP

【 摘 要 】

A complex of express methods for cyto-, geno-, and embryotoxicity assessment of nanoparticles (NPs), based on the simultaneous use of three models: the cultures of pre- and post-implantation embryos of mice (egg cleavage, blastocyst formation, 1-5 day of development) and rats (head fold-neurulation stages, 30 somite pairs, 9.5-11.5 day of development), as well as human peripheral blood lymphocytes, is developed. Pathogenic (dysmorphogenic) effects of SiO2 NPs in concentrations of 100 and 200 μg/mL on early embryo development processes were established. The cytogenetic methods used for cyto-, geno-, and mutagenicity assessment (micronuclear test under cytokinesis-block conditions, chromosomal aberrations test, and DNA comet assay) revealed no effects of SiO2 NPs in concentrations 200, 100, and 20 μg/mL.