Profile of gene expression regulated by induced p53: connection to the TGF‐β family

FEBS Letters

Amariglio, Ninette²  Rechavi, Gideon²  Givol, David¹  Kannan, Karuppiah¹ 

[1] Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel;Department of Pediatric Hematology–Oncology, The Chaim Sheba Medical Center and Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel

关键词: p53;    Transactivation;    Transrepression;    DNA array;    DNA repair;    MIC-1;   

DOI  :  10.1016/S0014-5793(00)01291-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The transcription regulatory function of p53 was analyzed by using two inducible p53 systems in the human lung cancer cell line H1299. cDNA probes derived from RNA harvested 12 h after p53 induction were used to probe filters containing cDNA arrays. Over 20 genes were found to be significantly induced or suppressed by p53. The induced genes can be classified mainly as cell cycle inhibitors like p21waf, GADD45, apoptosis-related genes like Fas/APO1 and PIG3 or DNA repair genes like DDB2, DNA ligase and G/T mismatch DNA glycosylase. The suppressed genes include mainly cell cycle regulators like cyclin B1, cyclin H and kinases like c-abl, CLK1 and others. The most notable induced gene was MIC-1, encoding a TGF-β-related secretory protein, suggesting a potential paracrine component for p53 growth suppression.

【 授权许可】

Unknown