Gene silencing by S‐adenosylmethionine in muscle differentiation

FEBS Letters

Fuso, Andrea¹  Orrù, Luigi¹  Cavallaro, Rosaria A.¹  Scarpa, Sigfrido¹  Buttarelli, Francesca R.² 

[1] Department of Cellular Biotechnologies and Hematology, c/o Research Laboratory, and Department of Surgical Sciences ‘P. Valdoni’, V.A. Scarpa 14, 00161 Rome, Italy;Department of Neurological Sciences, University of Rome ‘La Sapienza’, V. le Regina Elena 324, 00161 Rome, Italy

关键词: DNA methylation;    S-Adenosylmethionine cycle;    Transcription regulation;    Gene silencing;    Muscle differentiation;    GM;    growth medium (with 10% foetal calf serum);    DM;    differentiation medium (with 1% foetal calf serum);    CK;    creatine kinase;    SAM;    S-adenosylmethionine;    MM;    molecular weight marker;   

DOI  :  10.1016/S0014-5793(01)03030-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A well-characterised experimental system, the myogenin gene in C2C12 muscle cell culture, was chosen to better understand the methylation mechanism underlying the regulation of gene expression. We already demonstrated that demethylation dynamics of a specific CpG site in the 5′-flanking region of myogenin well correlates with gene expression and terminal differentiation. Here we demonstrate that S-adenosylmethionine-sulphate-p-toluenesulphonate (SAM) inhibits myogenin expression and myoblast differentiation by delaying the demethylation of specific CpG in differentiating myoblasts. These results suggest new perspectives in methylation mechanisms and the use of SAM in the partial silencing of gene expression, as it could be required in disease treatment.

【 授权许可】

Unknown