Gene silencing via DNA methylation in naturally occurring Tragopogon miscellus (Asteraceae) allopolyploids

BMC Genomics

Jennifer A Tate²  Pamela S Soltis¹  Douglas E Soltis¹  V Vaughan Symonds²  Tina Sehrish² 

[1] Florida Museum of Natural History, University of Florida, Gainesville, Florida, USA;Institute of Fundamental Sciences, Massey University, Palmerston North, New Zealand

关键词: Whole-genome duplication;    Tragopogon;    Gene silencing;    DNA methylation;    Allopolyploidy;   

Others  :  1216250
DOI  :  10.1186/1471-2164-15-701

 received in 2014-05-20, accepted in 2014-08-18,  发布年份 2014

【 摘 要 】

Background

Hybridization coupled with whole-genome duplication (allopolyploidy) leads to a variety of genetic and epigenetic modifications in the resultant merged genomes. In particular, gene loss and gene silencing are commonly observed post-polyploidization. Here, we investigated DNA methylation as a potential mechanism for gene silencing in Tragopogon miscellus (Asteraceae), a recent and recurrently formed allopolyploid. This species, which also exhibits extensive gene loss, was formed from the diploids T. dubius and T. pratensis.

Results

Comparative bisulfite sequencing revealed CG methylation of parental homeologs for three loci (S2, S18 and TDF-44) that were previously identified as silenced in T. miscellus individuals relative to the diploid progenitors. One other locus (S3) examined did not show methylation, indicating that other transcriptional and post-transcriptional mechanisms are likely responsible for silencing that homeologous locus.

Conclusions

These results indicate that Tragopogon miscellus allopolyploids employ diverse mechanisms, including DNA methylation, to respond to the potential shock of genome merger and doubling.

【 授权许可】

   
2014 Sehrish et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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