High resolution methylation analysis of the galectin‐1 gene promoter region in expressing and nonexpressing tissues

FEBS Letters

Chiariotti, Lorenzo¹  Caporaso, Milena¹  Salvatore, Paola¹  Bruni, Carmelo B¹  Benvenuto, Giovanna¹ 

[1] Centro di Endocrinologia ed Oncologia Sperimentale del Consiglio Nazionale delle Ricerche ``G. Salvatore', Dipartimento di Biologia e Patologia Cellulare e Molecolare ``L. Califano', Università degli Studi di Napoli ``Federico II', via S. Pansini 5, 80131 Naples, Italy

关键词: Galectin;    Promoter;    DNA methylation;    Transcription regulation;   

DOI  :  10.1016/S0014-5793(97)01553-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We conducted by bisulfite genomic sequencing a high resolution study of the methylation of the galectin-1 gene in expressing and nonexpressing tissues. We show that: (i) hypomethylation of galectin-1 promoter correlates with expression; (ii) differences in methylation occur in a small region, which include a CpG cluster; (iii) the density of methyl-CpGs rather than site-specific methylation distinguishes the nonexpressing from the expressing alleles; (iv) the modification profiles in nonexpressing tissues are highly heterogeneous; (v) a single CpG within 1300 bp is always methylated both in expressing and nonexpressing tissues; (vi) these features are conserved in rat and mouse.

【 授权许可】

Unknown