Evidence of a tissue‐restricting DNA regulatory element in the mouse IRBP promoter

FEBS Letters

Nguyen, H.T.²  Padove, S.A.²  Borst, D.E.³  Knox, B.E.¹  Stodulkova, E.²  Jones, K.M.¹  Boatright, J.H.²  Nickerson, J.M.² 

[1] SUNY Health Science Center, Syracuse, NY, USA;Emory Eye Center, Atlanta, GA, USA;Uniformed Service University of Health Science, Bethesda, MD, USA

关键词: Interphotoreceptor retinoid binding protein;    Transgenic Xenopus laevis;    Transcription regulation;    Promoter;    Repressive element;    Neuroblastoma;    Retinoblastoma;    CAT;    chloramphenicol acetyltransferase;    EGFP;    enhanced green fluorescent protein;    IRBP;    interphotoreceptor retinoid binding protein;    NRSE;    neuron-restrictive silencing element;    NRSF;    neuron-restrictive silencing factor;    HEK 293;    293 human embryonic kidney cells;   

DOI  :  10.1016/S0014-5793(01)02662-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The expression of interphotoreceptor retinoid binding protein (IRBP) is limited to photoreceptor cells of the retina and pinealocytes of the pineal gland. We sought to define cis-elements of the mouse IRBP 5′ flanking region that are required for this restricted activity. In vitro transient transfections of retinoblastoma and neuroblastoma cells and in vivo experiments with transgenic Xenopus laevis indicate that −1783/+101 and −156/+101 IRBP gene fragments directed expression predominantly to the retina and pineal, with minor neuronal expression elsewhere. In contrast, a −70/+101 fragment was less restrictive in controlling expression, exhibiting activity not only in retina, but also in forebrain, hindbrain, spinal cord, and motor neurons innervating gills.

【 授权许可】

Unknown