An in vitro DNA virus for in vitro protein evolution

FEBS Letters

Husimi, Yuzuru²  Nemoto, Naoto¹  Tabuchi, Ichiro²  Soramoto, Sayaka² 

[1] GenCom Co., 11 Minami-oya, Machida 194-8511, Japan;Department of Functional Materials Science, Saitama University, 255 Shimo-okubo, Saitama 338-8570, Japan

关键词: cDNA–protein fusion;    Evolutionary protein engineering;    In vitro selection;    DMSO;    dimethyl sulfoxide;    CPG;    controlled pore glass;    NTA;    nitrilotriacetic acid;    GFP;    green fluorescent protein;    OMe;    2′-O-methyl ribonucleotide;    WGE;    wheat-germ extract;   

DOI  :  10.1016/S0014-5793(01)03075-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In vitro virus is a molecular construct for in vitro protein evolution, which requires some mechanism to link phenotype to genotype. The first in vitro virus was realized by bonding a nascent protein with its coding mRNA via puromycin in in vitro translation. We report a new construct of in vitro DNA virus. The virion was a covalent cDNA–protein fusion, and virion formation did not require any modification of mRNA. Due to intactness of mRNA, this type of in vitro DNA virus will take the next step toward in vitro autonomous evolution, just like in vivo viral evolution in a cellstat.

【 授权许可】

Unknown