Identification of a structural motif of 23S rRNA interacting with 5S rRNA

FEBS Letters

Cho, Bongrae³  Lee, Younghoon¹  Park, Inwon²  Ko, Jaehyeong¹ 

[1] Department of Chemistry and Center for Molecular Design and Synthesis, Korea Advanced Institute of Science and Technology, Taejon 305-701, South Korea;Department of Chemistry, Seoul National University, Seoul 151-742, South Korea;Department of Chemistry, Chongju University, Chongju 360-764, South Korea

关键词: 5S rRNA;    Systematic evolution of ligands by exponential enrichment;    Aptamer;    23S rRNA;    SELEX;    systematic evolution of ligands by exponential enrichment;    EF-G;    elongation factor-G;   

DOI  :  10.1016/S0014-5793(01)03068-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To identify RNA motifs interacting with 5S rRNA, a systematic evolution of ligands by exponential enrichment experiment was applied. Some of the resulting RNA aptamers contained a consensus sequence similar to the sequence in the loop region of helix 89 of 23S rRNA. We show that the synthetic helix 89 RNA motif indeed interacted with 5S rRNA and that the region around loop B of 5S rRNA was involved in this interaction. These results suggest the presence of a novel RNA–RNA interaction between 23S rRNA and 5S rRNA which may play an important role in the ribosome function.

【 授权许可】

Unknown