Initial analysis of 750 MHz NMR spectra of selectively 15N‐G,U labelled E. coli 5S rRNA

FEBS Letters

Soskic, Vukic²  Brown, Larry R.²  Bald, Rolf¹  Erdmann, Volker A.¹  Fürste, Jens Peter¹  Görlach, Matthias²  Grüne, Matthias²  Klussmann, Sven¹ 

[1] Institut für Biochemie, Freie Universität Berlin, Thielallee 63, D-14195 Berlin, Germany;Institut für Molekulare Biotechnolgie e. V., Posifach 100813, D-07708 Jena, Germany

关键词: NMR;    15N labelling;    Ribosome;    5S rRNA;    E-domain;    E. coli;   

DOI  :  10.1016/0014-5793(96)00361-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The overall folding of an RNA molecule is reflected in its base pairing pattern. The identification of that pattern provides a first step towards the determination of the structure of an RNA molecule. We show that the application of heteronuclear NMR methods at 750 MHz to E. coli 5S rRNA (120 nucleotides) selectively labelled with ¹⁵N in guanine and uridine allows observation of base pairing patterns for a larger RNA molecule. We also present evidence that the fold of the E-domain of the 5S rRNA (nt 79–97) as a contiguous part of the 5S rRNA and as an isolated molecule is virtually the same.

【 授权许可】

Unknown