Crystal structure of domain E of Thermus flavus 5S rRNA: a helical RNA structure including a hairpin loop

FEBS Letters

Betzel, Christian¹  Lorenz, Siegfried²  Erdmann, Volker A²  Nolte, Alexis²  Perbandt, Markus²  Bald, Rolf² 

[1] Institut für Physiologische Chemie, Universitäts-Krankenhaus Eppendorf, Arbeitsgruppe für Makromolekulare Strukturanalyse, c/o DESY Geb. 22a, Notkestraße 85, 22603 Hamburg, Germany;Institut für Biochemie, Freie Universität Berlin, Thielallee 63, 14195 Berlin, Germany

关键词: 5S rRNA;    Crystal structure;    RNA-RNA interaction;    Hairpin loop;   

DOI  :  10.1016/S0014-5793(98)00436-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The synthetic RNA fragment 5′-CUGGGCGG(GCGA)CCGCCUGG (nucleotides in parentheses indicate the loop region) corresponds to the natural sequence of domain E from nucleotides 79–97 of the Thermus flavus 5S rRNA including a hairpin loop. The RNA structure determined at 3.0 Å and refined to an R-value of 24.1% also represents the first X-ray structure GNRA tetraloop. The loop is in distinctly different conformation from other GNRA tetraloops analyzed by NMR. The conformation of the two molecules in the asymmetric unit is influenced and stabilized by specific intermolecular contacts. The structural features presented here give evidence for the ability of RNA molecules to adapt to specific environments.

【 授权许可】

Unknown