Stable expression of biologically active recombinant bovine interleukin‐4 in Trypanosoma brucei

FEBS Letters

Jungi, T.W.²  Roditi, I.¹  Furger, A.¹  Salomone, J.-Y.¹  Weynants, V.³ 

[1] Institut für Zellbiologie, Universität Bern, Baltzerstrasse 4, CH-3012 Bern, Switzerland;Institut für Veterinär Virologie, Universität Bern, Länggassstrasse 122, CH-3012 Bern, Switzerland;URBM, Laboratoire d'Immunologie-Microbiologie, Faculté Universitaires, Notre-Dame de la Paix, 61 rue de Bruxelles, 5000 Namur, Belgium

关键词: Expression system;    Bioassay;    Interleukin;    Cytokine;    Growth factor;    Recombinant protein;    FBS;    foetal bovine serum;    GARP;    glutamic acid/alanine-rich protein;    PBMC;    peripheral blood bovine mononuclear cells;    (r)boIL-4;    (recombinant) bovine interleukin-4;    (r)huIL-4;    (recombinant) human interleukin-4;    UTR;    untranslated region;   

DOI  :  10.1016/S0014-5793(01)03031-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have explored the potential of Trypanosoma brucei as a eukaryotic expression system. Procyclic forms, which correspond to an insect-adapted stage, can easily be cultured in vitro. The cells grow to densities ∼10-fold greater than higher eukaryotic cells and are not infectious for mammals. An expression vector which can stably integrate into the genome was used to express high levels of recombinant bovine interleukin-4 (IL-4). Trypanosome-derived IL-4 is released into the medium and is biologically active. The recombinant protein down-regulates CD14 expression in human macrophages and inhibits NO production by stimulated bovine macrophages.

【 授权许可】

Unknown